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Overview
Who: John SK Yuen Jr., Brigid M Barrick, Hailey DiCindio, Jaymie A Pietropinto, and David L Kaplan
Published: June 16, 2023
Where: ACS Biomaterials Science & Engineering
Key Takeaway: Growing muscle and vascular cells together requires optimization
Research Topics:
cell
media
Summary
In this study, John Yuen et al generate 3D muscle tissues capable of supporting both muscle and endothelial cells. Endothelial cells form blood vessels and are likely necessary for growing thick 3D tissues for cultured meat. In this study, the investigators tested media formulations able to support both muscle and endothelial cells. In addition, the ratio of muscle to endothelial cells is studied to find the best combination of media and cell ratios to support endothelial cell survival and muscle differentiation. The authors find that the optimal cultured media formulation for 2D culture does not necessarily translate to 3D culture, highlighting the need for more research on 3D tissues. Future work will need to assess if the endothelial cells are able to form tubes to serve as vessels for media perfusion.
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Funding
Cite This Publication
Yuen Jr, J. S., Barrick, B. M., DiCindio, H., Pietropinto, J. A., & Kaplan, D. L. (2023). Optimization of Culture Media and Cell Ratios for 3D In Vitro Skeletal Muscle Tissues with Endothelial Cells. ACS Biomaterials Science & Engineering.
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