Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture

Overview

Who: Cameron Semper, Alexei Savchenko

Published: June 13, 2023

Where: STAR Protocols

Key Takeaway: A step-by-step protocol for producing low-cost growth factors for cultured meat

Research Topics: media

Summary

Cameron Semper and Alexei Savchenko present a workflow for the production of growth factors in E. coli. Specifically, the protocol produces mitogenic growth factors: signaling proteins that are critical for cell proliferation, migration, and differentiation. These proteins are particularly challenging to produce, typically requiring complex or expensive processes. This protocol presents a simple and low-cost process to produce a variety of growth factors.

To see this protocol in action, check out Recombinant production of growth factors for application in cell culture.

Go to Publication

Funding

Cite This Publication

Semper, C., & Savchenko, A. (2023). Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture. STAR protocols, 4(3), 102351.

Copied!