A simple and robust serum-free media for the proliferation of muscle cells

Overview

Who: Stig Skrivergaard, Jette Feveile Young, Navid Sahebekhtiari, Cameron Semper, Meenakshi Venkatesan, Alexei Savchenko, Peter J. Stogios, Margrethe Therkildsen, Martin Krøyer Rasmussen

Published: July 8, 2023

Where: Food Research International

Key Takeaway: Tri-basal 2.0+ media is a simplified serum-free media that can sustain bovine satellite cells

Research Topics: media

Summary

In this article, Stig Skrivergaard et al design an optimized serum-free media for bovine satellite cells with as few ingredients as possible, named Tri-basal 2.0+. Media development is challenging because of the number of possible combinations of ingredients and concentrations. To increase efficiency, the authors use Design of Experiments and Response Surface Methodology in combination with high-throughput imaging to screen a large number of possible combinations. Their work identifies just 4 critical components (fetuin, albumin, insulin-transferrin-selenium and recombinant FGF2) that can sustain cell proliferation at a similar level as 10% fetal bovine serum. The authors acknowledge that the medium cost is still too high to support large-scale cell culture, and future work should focus on scalable production or replacement of high-concentration proteins.

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Skrivergaard, S., Young, J. F., Sahebekhtiari, N., Semper, C., Venkatesan, M., Savchenko, A., ... & Rasmussen, M. K. (2023). A simple and robust serum-free media for the proliferation of muscle cells. Food Research International, 172, 113194.

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