Simple and Effective Serum-Free Medium for Sustained Expansion of Bovine Satellite Cells for Cell Cultured Meat

Overview

Who: Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen Jr., David L. Kaplan

Published: June 2, 2022

Where: Communications Biology

Key Takeaway: Beefy-9, a derivative of serum-free growth media B8, provides a starting point in developing scalable, economical serum-free growth media for cultured meat production.

Research Topics: media

Summary

Andrew Stout et al. propose modification of B8 serum-free growth media to create Beefy-9, a serum-free growth media specific to bovine satellite cells. To optimize the formulation, B8 is supplemented with assorted concentrations and combinations of compounds relevant to bovine satellite cell growth. Stout et al. find recombinant human albumin (rAlbumin) can improve cell growth to a significantly greater degree than any other supplement tested, and create Beefy-9 by adding 800 μg/mL rAlbumin to B8. Next, Beefy-9 is tested in cell growth and differentiation, and is shown to significantly increase cell growth and differentiation compared to B8. Overall, Stout et al. argue Beefy-9 is a relatively low-cost, simple starting point in developing an ideal serum-free media for cultured meat production, though they recommend further optimization to reduce cost further.

Written by Morgan Ziegelski

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Stout, A. J., Mirliani, A. B., Rittenberg, M. L., Shub, M., White, E. C., Yuen, J. S. K., & Kaplan, D. L. (2022). Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat. Communications Biology, 5(1), 466. https://doi.org/10.1038/s42003-022-03423-8

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