The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production

Overview

Who: Edward N. O'Neill , Milla Neffling, Nick Randall, Grace Kwong, Joshua Ansel, Keith Baar, David E. Block

Published: March 31, 2023

Where: Future Foods

Key Takeaway: A comparison of how cells use nutrients in serum vs serum-free media

Research Topics: media

Summary

In this publication, Edward O’Neill et al analyze spent media, or media after cells have grown in it, to study how cells use key ingredients in the media over time. This type of analysis is helpful to understand the nutritional needs of cells and ensure media is meeting those needs most efficiently. The authors compared media with 10% FBS and E8, a common serum-free media, to better understand how to replace serum. They find that cells use glucose (or sugar) and produce waste products similarly in both media types, indicating that the absence of serum does not affect glucose metabolism. FGF2, a key growth factor, is also used similarly, despite very different starting amounts in each media. However, several key amino acids are utilized differently. The authors identify multiple areas of future work to improve cell and media efficiency.

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Cite This Publication

O'Neill, E. N., Neffling, M., Randall, N., Kwong, G., Ansel, J., Baar, K., & Block, D. E. (2023). The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production. Future Foods, 7, 100226.

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